Alzheimer’s risk factor FERMT2 promotes the progression of colorectal carcinoma via Wnt/β-catenin signaling pathway and contributes to the negative correlation between Alzheimer and cancer
Fig 5
Knockdown of FERMT2 inhibited cell proliferation and colony formation in CRC cells.
(A, B) The FERMT2 mRNA (A) and protein (B) expression levels in five CRC cell lines and normal colon epithelial cells were detected by RT-qPCR and western blot, respectively. (C-F) The silencing effects on FERMT2 by shRNA in SW1116 (C and D) and SW1463 (E and F) cells were evaluated by RT-qPCR and western blot assay, respectively. (G-H) Effects of FERMT2 silencing on cell proliferation viability in SW1116 and SW1463 cells were measured using a CCK-8 cell-counting kit. (I, J) The colony formation potential of SW1116 and SW1463 cells after FERMT2 knockdown were analyzed with a colony formation assay. All the tests were repeated at least three times. *P < 0.05, **P < 0.01, ***P < 0.001 vs shNC.