TIPRL Inhibits Protein Phosphatase 4 Activity and Promotes H2AX Phosphorylation in the DNA Damage Response
Fig 5
Overexpression of TIPRL promotes cell death in response to genotoxic stress.
(A) 3T3 MEFs stably expressing LPC FLAG vector control (VEC) or LPC FLAG-TIPRL (FLAG-TIPRL) were lysed and immunoblotted with the indicated antibodies. (B) Cells were treated with 10μM CPT or 2μg/ml doxorubicin for 24hrs. Viability was measured by propidium iodide exclusion. Data represent ± standard deviation of the mean of three independent experiments. (C) 3T3 MEFs stably expressing LPC FLAG vector control (VEC) or LPC FLAG-TIPRL (FLAG-TIPRL) were treated with the indicated concentration of doxorubicin (DOXO) for 24hrs. Cell viability was measured by MTS assay. (D) 3T3 cells stably expressing LPC FLAG vector control (VEC) or LPC FLAG-TIPRL (FLAG-TIPRL) were treated with the indicated concentration of doxorubicin (DOXO) for 24hrs. Cells were lysed and immunoblotted with the indicated antibodies. (E) 3T3 MEFs stably expressing LPC FLAG vector control (VEC) or LPC FLAG-TIPRL (FLAG-TIPRL) were treated with the indicated concentration of CPT for 24hrs. Cells were lysed and immunoblotted with the indicated antibodies. **p<0.005, ***p<0.001, Student’s t test.