MicroRNA-181a/b-1 Is Not Required for Innate γδ NKT Effector Cell Development
Fig 5
Dendritic epidermal T cells develop in the absence of miR-181a/b-1.
(A) Analysis of Vγ5+ γδ T cells in the skin of miR-181a/b-1–/–x TcrdH2BeGFP mice (–/–) compared to TcrdH2BeGFP mice (ctrl.). (left) Representative contour plots illustrating the gating for Vγ5+ γδ T cells. (right) Scatter plot shows frequencies of Vγ5+ γδ T cells, pooled data from two independent experiments with each n = 3 mice per group, mean. (B) Histological analysis of epidermal sheets from ears of miR-181a/b-1–/–x TcrdH2BeGFP mice (–/–) compared to TcrdH2BeGFP mice (ctrl.). Epidermal sheets were stained for DETCs (yellow, overlay red and green) with CD3 (red) and TcrdGFP+ (green) cells indicate γδ T cells. Original magnification: 20x, scale bars: 20μm.