Label-Free Protein-RNA Interactome Analysis Identifies Khsrp Signaling Downstream of the p38/Mk2 Kinase Complex as a Critical Modulator of Cell Cycle Progression

doi:10.1371/journal.pone.0125745

Label-Free Protein-RNA Interactome Analysis Identifies Khsrp Signaling Downstream of the p38/Mk2 Kinase Complex as a Critical Modulator of Cell Cycle Progression

Fig 5

Khsrp-dependent regulation of Cdkn1a^P21.

(A) Cell cycle analysis and (B) mitotic index of wildtype and Khsrp^-/- MEFs expose a Khsrp-dependent (A) accumulation of cells in G₁ and (B) decreased cycling rates. (C) Transcript and (D) protein levels of Cdkn1a^P21 in Khsrp^-/- cells measured by qPCR and immunoblotting reveals an increase of Cdkn1a^P21 protein levels unrelated to Cdkn1a^P21 mRNA levels.

doi: https://doi.org/10.1371/journal.pone.0125745.g005