Reduction of the HIV Protease Inhibitor-Induced ER Stress and Inflammatory Response by Raltegravir in Macrophages
Figure 5
Effect of raltegravir on HIV PI-induced lipid accumulation in mouse J774A.1 macrophages.
(A) J774A.1 cells were loaded with Acetylated-LDL (50 µg/ml) and treated with 25 µM of LOPV, RITV, or vehicle control with or without raltegravir (25 µM) for 24 h. The intracellular lipid was stained with Oil Red O as described under “Methods”. The images were taken with the use of an Olympus microscope equipped with an image recorder. The representative images are shown for each treatment group. (B). J774A.1 cells were loaded with oxidized-LDL (50 µg/ml) and treated with 25 µM of LOPV/RITV (4∶1), or vehicle control with or without raltegravir (25 µM) for 24 h. The intracellular lipid was stained with Nile Red as described under “Methods”. Images were obtained under a 40×objective using a FITC filter on a fluorescent microscope. The representative images are shown for each treatment group.