The Attenuated Brucella abortus Strain 19 Invades, Persists in, and Activates Human Dendritic Cells, and Induces the Secretion of IL-12p70 but Not IL-23
Figure 5
B. abortus strain S19 persists in less mature cells in both medium-incubated and cytokine-matured DCs.
Immature DCs were infected with B. abortus S19 at MOI 20, the bacteria were washed out, antibiotics were added, and the cells were incubated in the presence or absence or pro-inflammatory cytokines. At days 2, 6, and 10, cells was harvested and analyzed by flow cytometry for their extracellular expression of CD25 and the intracellular content of Brucella-LPS. One representative experiment of three independent experiments is shown.