The SHOCT Domain: A Widespread Domain Under-Represented in Model Organisms
Figure 5
The SHOCT peptide does not multimerise the rat Cd4d3+4 protein.
(A) Tissue culture supernatants containing biotinylated Cd4d3+4-F0QBY7.1 and Cd4d3+4-F0QBY7.1_shuffled were resolved by SDS-PAGE under reducing conditions, blotted and detected using streptavidin-HRP. (B) Purified Cd4d3+4-F0QBY7.1 and Cd4d3+4-F0QBY7.1_shuffled were resolved on a Superdex 2000 Tricorn 10/600 column. The elution volumes of protein standards are marked in red. The expected monomer size for Cd4d3+4-peptide is 33 kDa. (C) Purified Cd4d3+4-F0QBY7.1 and Cd4d3+4-F0QBY7.1_shuffled were resolved by SDS-PAGE under non-reducing conditions before (Ni) and after size exclusion chromatography (SE) and detected Coomassie Brilliant Blue R-250 staining.