APRIL Induces Tumorigenesis and Metastasis of Colorectal Cancer Cells via Activation of the PI3K/Akt Pathway
Figure 5
APRIL regulates the cell cycle and cell cycle regulatory proteins of SW480 cells through PI3K/Akt pathway.
Cell cycle profiles of shAPRIL (sh637) transfected SW480 cells (A), or rhAPRIL stimulated SW480 cells (B) were examined by flow cytometry with propidium iodide staining and cell numbers were counted according to DNA content of G0/G1, S and G2/M phases. The cell cycle distribution was from three independent experiments, and shows the percentage of G0/G1 and S+G2/M cells after shAPRIL transfection or rhAPRIL stimulation. Western blot of c-myc, cyclin D1, CDK4, p-Rb, p-Akt, p-mTOR and β-actin in SW480 cells 48 h after shAPRIL transfection (C) or rhAPRIL stimulation (D). SW480 cells were treated with the indicated doses of LY294002 (E) or rapamycin (F) for 24 h, and then stimulated with rhAPRIL (400 ng/ml) for 12 h, and analyzed for expression of c-myc, cyclin D1, CDK4, p-Rb. Densitometric values are listed below each blot.