Downregulation of the AU-Rich RNA-Binding Protein ZFP36 in Chronic HBV Patients: Implications for Anti-Inflammatory Therapy
Figure 5
Reduced expression of ZFP36 in PBMCs after treatment with HBsAg, IL-8, RANTES, or IFN-γ.
A. ZFP36 expression in PBMCs cultured with HBsAg (2 µg/ml), IL-8 (40 pg/ml), RANTES (30 ng/ml), or IFN-γ (1 ng/ml) for 20 hours. Shown are ZFP36 mRNA expression levels of samples from six healthy donors as measured by qPCR. B. Increased production of IL-8 and RANTES but not IFN-γ by HBsAg-stimulated PBMCs. PBMCs were cultured and tested as in A. C. Effect of bisindolylmaleimide I on IL-8-induced ZFP36 downregulation in PBMCs. PBMCs were cultured with IL-8 as in A in the presence of PKC inhibitor (BIS), PKA inhibitor (H89), or p38MAPK inhibitor (SB 203580). The expression of ZFP36 was measured by qPCR. *p<0.05; ** p<0.01; *** p<0.001.