MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines
Figure 5
Confirmation of mRNA down-regulation by qRTPCR for predicted targets.
A) Representative nucleotide sequence matches between possible target genes and miRNAs. The numbers in parenthesis indicates the positions of target nucleotides from the stop codon. Only matched nucleotides with miRNA seed sequences are indicated with the vertical lines. B) The quantitative RT-PCR analyses of potential targets of miR-107 (CCNE1, CDK6, CDCA4, RAB1B and CRKL) and miR-185 (CCNE1, CDK6, AKT1, HMGA2, CORO2B) are shown. The vertical axis indicates the relative expression ratio of each gene normalized with that of GAPDH. C) Western Blot showing down-regulation of CDK6 protein by miR-107.