Potent and Broad Inhibition of HIV-1 by a Peptide from the gp41 Heptad Repeat-2 Domain Conjugated to the CXCR4 Amino Terminus
Fig 5
Expression of C34-conjugated coreceptors inhibits HIV-1 in primary CD4 T cells.
Primary human CD4 T cells either untransduced or transduced with the indicated C34-conjugated constructs or GFP control, were either inoculated or not inoculated with HIV-1 JRFL, and monitored by flow cytometry for C34 peptide surface expression and intracellular HIV-1 p24-Gag. (A) Expression of C34-coreceptors or C34-CD4 in cells cultured in the absence of HIV-1 inoculation at Days 0, 5 and 14. (B) Cells inoculated with HIV-1 JRFL show stable expression of C34-constructs at Days 5 and 14 with marked inhibition of p24-Gag expression in C34-CCR5 and C34-CXCR4 transduced cultures relative to untransduced, GFP- or C34-CD4 transduced cells. (C) Cells transduced with C34-CCR5, C34-CXCR4, C34-CD4 or GFP were added at a 1:3 ratio to untransduced cells for a final percentage of 25% transduced cells and inoculated with JRFL. Expansion of cells expressing C34-CCR5 or C34-CXCR4 is shown over time. No expansion of the C34-CD4- or GFP-transduced cells was seen. A representative experiment from 2 independent experiments is shown, each with a different healthy donor.