Parasite Fate and Involvement of Infected Cells in the Induction of CD4+ and CD8+ T Cell Responses to Toxoplasma gondii
Figure 5
Dendritic cells are required for optimal CD4+ and CD8+ T cell responses.
CD11c-DTR mice were administered diphtheria toxin 1 day prior to cpsII-OVA vaccination. At the time of vaccination, some mice were sacrificed to determine the efficiency of depletion. Percentages and numbers of dendritic cells from the spleen are shown. FACS plots are gated on CD3−,CD19−,NK1.1− cells (a). Eight days following vaccination, mice were sacrificed and total and tetramer-specific CD4+ and CD8+ T cell responses were analyzed. Total CD4+ T cell responses from the spleens are shown (b, top). Tetramer-specific CD4+ T cell responses from pooled lymph nodes and splenocytes were determined in a separate experiment (b, bottom). Flow plots are gated on CD4+ T cells (b), and the population examined was magnetically enriched for the tetramer+ve population (b, bottom). Total and OVA-specific CD8+ T cell responses from the PECS are depicted (c), and flow plots are gated on CD8+ T cells. Significant differences in tetramer and total CD8+ T cell responses between WT and CD11c-DTR mice were also apparent in the spleen. *p<0.05; **p<0.005. AVG±SE.