Association of Human TLR1 and TLR6 Deficiency with Altered Immune Responses to BCG Vaccination in South African Infants
Figure 5
Two TLR polymorphisms are associated with increased expression of cytotoxic molecules after ex vivo BCG stimulation.
Whole blood drawn 10 weeks after BCG at birth was re-stimulated with BCG ex vivo for 3 days. Granulysin, granzyme B and perforin production were measured by intracellular cytokine staining in (A) CD4-positive and (B) CD8-positive T cells. A general linear model was used to examine whether 4 TLR polymorphisms (TLR1_T1805G, TLR1_A1188T, TLR6_G1083C, and TLR6_C745T) were associated with BCG-induced cytotoxicity markers after subtraction of unstimulated control values. *P<0.05, **P<0.01.