Aqueous Extract of Gracilaria tenuistipitata Suppresses LPS-Induced NF-κB and MAPK Activation in RAW 264.7 and Rat Peritoneal Macrophages and Exerts Hepatoprotective Effects on Carbon Tetrachloride-Treated Rat
Figure 5
Effect of AEGT on MAP kinase phosphorylation in LPS-stimulated RAW 264.7 cells.
The RAW264.7 cells were seeded in a 24-well plate, treated with 400 or 800 µg/ml AEGT for 1 h and stimulated with 1 µg/ml LPS for 24 h. The cell lysates were collected, and p38, JNK, and ERK1/2 phosphorylation was analyzed by western blot analysis with specific antibodies. The relative value of the LPS- group or AEGT/LPS-treated group over the untreated group was measured by densitometry following normalization to cellular GAPDH levels. The basal level of promoter activity in the AEGT/LPS-untreated cells was defined as 1. Error bars indicate the means ± SD of three independent experiments. *P<0.05; **P<0.01.