Generation of Mouse Small Intestinal Epithelial Cell Lines That Allow the Analysis of Specific Innate Immune Functions
Figure 5
Stimulation of intestinal epithelial interferon reporter cell line by viral and bacterial infection.
(A) mx2 induction in IEC-Mx2Luc-10 cells upon infection with 80 HAU/ml Newcastle disease virus (NDV) for 24 h (n=3). (B) mx2 induction in IEC-Mx2Luc-10 cells upon infection with Salmonella Typhimurium and Listeria monocytogenes at a multiplicity of infection (MOI) of 10-20 or 100-200 for 74 h (n=4) and quantification of type I/III IFN secretion upon bacterial infection by incubation of IEC-Mx2Luc-10 cells with filtered conditioned medium from bacterially infected cells for 24 h (n=3). Fold induction represents relative luminescence units (RLU) of infected/stimulated compared to untreated cells. All values are shown as mean ± SD. Statistics were calculated with the unpaired Student’s t-test (****P<0.0001).