Genetic Variants and Increased Expression of Parascaris equorum P-glycoprotein-11 in Populations with Decreased Ivermectin Susceptibility
Figure 5
Expression of pgp-11 and pgp-16 mRNAs in pre-adult Parascaris equorum.
A randomly chosen group (n = 5) was compared with a group where ML treatment failed (n = 5). As a reference, expression of the actin, gpd-1 and 18S rRNA genes was measured. Real-time RT-PCR was conducted in duplicate for each sample using two different cDNAs for each biological replicate. Differences between randomly selected and putatively resistant worms were identified with Mann-Whitney U tests. **, p<0.01; *, p<0.05; #, p<0.056.