Role of N-Terminal His6-Tags in Binding and Efficient Translocation of Polypeptides into Cells Using Anthrax Protective Antigen (PA)
Figure 5
Voltage dependency of PA63-channels in the presence of EDIN and His6-EDIN.
A: Current response of PA63-channels in presence of EDIN. Voltage pulses between +20 and +70 mV were applied to a diphytanoyl phosphatidylcholine/n-decane membrane in the presence of PA63-pores and EDIN (both added only to the cis side of the membrane). The aqueous phase contained 150 mM KCl, 10 mM MES-KOH, pH 6. The temperature was 20°C. B: Current response of PA63 channels in the presence of His6-EDIN. Voltage pulses between +10 and +90 mV were applied to a diphytanoyl phosphatidylcholine/n-decane membrane in the presence of PA63-pores and His6-EDIN (both added only to the cis side of the membrane). The aqueous phase contained 150 mM KCl, 10 mM MES-KOH, pH 6. The temperature was 20°C. Note the change of the scale (Arrow).