Anti-schistosomal Intervention Targets Identified by Lifecycle Transcriptomic Analyses
Figure 5
Markov clustering expands the repertoire of differentially expressed transcripts associated with the schistosome dioecious and digenetic state.
Network analysis was performed as described in Methods (Pearson correlation, r = 0.94 and Markov cluster stringency = 1.7). Selected MCLs are included to illustrate transcripts enriched in the intermediate host (A, MCL9), definitive host (B, MCL69) or in adult male and female schistosomes (C, MCL7 - Male > Female; MCL12 - Female > Male). In each case, specific examples of transcripts included in the selected MCLs are listed (full list of transcripts included in each cluster is found in Dataset S6). Enriched Gene Ontology Molecular Function categories are also included where identified. Bar graphs represent average normalised AF647 intensity values (Dataset S4) for all transcripts in that cluster, with replicate values obtained from independent life-stage hybridizations indicated as identical colours.