Oncogenic Kras Initiates Leukemia in Hematopoietic Stem Cells
Figure 5
Transplanted KrasG12D Flk2− LSK Cells Induce MPD
Mice were analyzed 3 mo after transplantation with either KrasG12D or WT Flk2− LSK cells for evidence of MPD (n = 3 WT, n = 6 KrasG12D in a single experiment).
(A) Numbers of circulating leukocytes (WBC).
(B) Spleen weights.
(C) Percentages of myeloid cells (Mac1+ and/or Gr1+) in the spleen.
(D) Percentages of erythroid precursors (nucleated and TER119+) in the spleen.
(E) A representative splenic section demonstrating infiltration by mature myeloid cells (ring-shaped nuclei) and erythroblasts (small dense nuclei).