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Dexamethasone treatment of murine auditory hair cells and cochlear explants attenuates tumor necrosis factor-α-initiated apoptotic damage

Fig 4

Attenuation of TNF-α-induced apoptosis by DEX pretreatment.

Cells were pretreated with DEX for 6 h before exposure to TNF-α for 24 h. (A) Representative images of Annexin V (green) and PI (red) staining in HEI-OC1 cells. Scale bar = 100 μm, Original magnification = 100 ×. (B) Values in the graphs are presented the percentage of apoptotic cells. The data in graphs are expressed as means ± SE (n = 3). *,# P < 0.05, ** P < 0.01, *** P < 0.001, *,**,***; compared with untreated control, #; TNF-α only versus DEX plus TNF-α.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0291780.g004