Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves
Fig 4
Myrosinase activity, myrosinase protein abundance and nitrilase activity in rosette leaves after extended darkness (ED).
The plants were transferred to complete darkness for 3 d (3d ED) or 7 d (7d ED) after six weeks of growth under short day conditions. Myrosinase (A) as well as nitrilase (C) activity was measured photometrically (error bars show the standard deviation of five to 19 biological replicates). For protein abundance of TGG1 and TGG2 (B), immunoblotting and normalization to the control was performed (error bars represent the standard deviation of three biological replicates from three independently performed Western blots). Asterics indicate significant differences (Student’s T-test; P < 0.05) compared to the control.