Endoglin inhibition leads to intussusceptive angiogenesis via activation of factors related to COUP-TFII signaling pathway
Fig 4
Real-time RT-PCR assessment of ENG expression and gene expression alterations of TGFβ and ENG downstream signaling molecules and transcription factors.
a: After treatment with anti ENG specific antibody the ENG gene expression was significantly down-regulated after 6 h, 12 h in comparison to the controls without treatment (dashline). After 24 h gene expression was almost restored to basic level. Repeated application of ENG specific antibody led to dramatically decreased expression around 48h after. b and c: Upregulated expression of three primary of types TGFβ as well BMP7 and follistatin (FST). d and g: Gene expression profile of SMADs proteins 1–7 in time-course after ENG inhibition. e:, BMP-related molecules ACVR1 and ACVR2A as well as bone morphogenic proteins 2 and 9-epression profiles demonstrated reduced expression after ENG inhibition. f: TGFβR1 (ALK5) and TGFβR3 factors interacting with ENG were down-regulated. h: ENG downstream signaling molecules NOG, EMP1, CREBBP and ID1 showed significant decreased expression. Usually Notch cooperating SMAD3 expression was down-regulated (d) also in comparison to SMAD6 (g). All: pooled sampling (n = 5), analyzed in triplicate.