Complete Genome Sequence of Borrelia afzelii K78 and Comparative Genome Analysis
Fig 4
Species-specific variation of the intergenic variable region in the circular plasmid cp26.
The variable sequence segment in the cp26 plasmids of K78 is compared with 25 Borrelia strains. A maximum likelihood tree rooted with a B. bissettii sequence as outgroup, shows the relationship for the intergenic part, which in B. afzelii K78 is situated between BAFK78_A0014 and BAFK78_A0015 (bacterial extracellular solute-binding protein), and which shows a species-specific length. Insertions and deletions within this region have been analyzed with the program Mauve, and the compositional analysis for 16 of the 26 sequences (underlined in the tree view) is shown with related segments marked by color and/or boxes together with a similarity score diagram for each sequence. Blue bars denote segments which, in some strains, have been annotated as short hypothetical proteins (the number of assigned proteins in this region is indicated in parentheses in the tree view).