Generation of Mouse Small Intestinal Epithelial Cell Lines That Allow the Analysis of Specific Innate Immune Functions
Figure 4
Interferon response of intestinal epithelial interferon reporter cell line.
(A) Stimulation of IEC-Mx2Luc-10 cells with indicated amounts of IFN-β or IFN-λ3 for 24 h. Fold induction represents relative luminescence units (RLU) of treated compared to untreated cells. All values are depicted as mean ± SD (n=3). (B) Antiviral protection of IEC-Mx2Luc-10 cells upon treatment with IFN-β and IFN-λ3. Cells were pre-treated with 1000 U/ml IFN-β or 50 ng/ml IFN-λ3 for 24 h and infected with 80 HAU/ml Newcastle disease virus (NDV) for 24 h. Cells were stained for expression of NDV hemagglutinin-neuraminidase (HN) and analyzed by flow cytometry.