The Attenuated Brucella abortus Strain 19 Invades, Persists in, and Activates Human Dendritic Cells, and Induces the Secretion of IL-12p70 but Not IL-23
Figure 4
Prolonged intracellular survival of B. abortus S19 in unstimulated DCs compared with cytokine-matured DCs.
Immature DCs were infected with B. abortus S19 at MOI 20, the bacteria were washed out, antibiotics were added, and the cells were then incubated in the presence or absence or pro-inflammatory cytokines. Cells were lysed at day 0 to determine the initial number of intracellular bacteria, and on days 2, 6, and 10 to ascertain the intracellular bacterial numbers over time. CFUs differed significantly at d10 (*, p<0.05).