Loss of Atrx Sensitizes Cells to DNA Damaging Agents through p53-Mediated Death Pathways
Figure 4
Atrx-null cells have a general sensitivity to DNA damaging agents.
(A) Representative images of TUNEL positive neurons (red) isolated from wild type (WT) or Atrx-deficient (KO) mice following 5-FU treatment. Cells were counterstained with DAPI (blue). DNase I treatment (DNaseI) served as a positive control for TUNEL staining. Untreated cells served as a negative control (-ve Ctrl). Scale bar: 50 mM. Quantification of TUNEL positive neurons is depicted in the graph on the right. (B, C) Primary myoblasts (B) or MEFs (C) isolated from Atrxf/f mice were untreated (grey bar) or treated with 5-FU after infection with AdLacZ (white bar) or AdCre (black bar). Mean values are plotted with the bars corresponding to 95% confidence intervals. Asterisks denote statistically significant differences (p≤0.05). (D, E) Primary myoblasts (D) or MEFs (E) isolated from Atrxf/f mice were treated with cisplatin (20 mM, 24 hrs) or UV light (10 J/m2) after infection with AdLacZ (white bar) or AdCre (black bar). Mean values are plotted with the bars corresponding to 95% confidence intervals. Asterisks denote statistically significant differences (p≤0.05).