Regulation of CD4+ and CD8+ Effector Responses by Sprouty-1
Figure 4
Spry1 null CD8+ T cells have enhanced effector function.
Spry1 null and wild type spleens were expanded in vitro with anti-CD3, spleens were rechallenged with anti-CD3. A. CFSE dilution of wild type and Spry1 null CD8+ T cells. Intracellular cytokine secretion assay was performed for IFNγ (B) and Granzyme-B (C). A mixture of CFSE high labeled, peptide pulsed splenocytes and CFSE low unlabeled splenocytes were injected I. V. into either wild type or Spry1Flox/Flox Lck Cre mice. Sixteen hours later splenocytes were analyzed by flow cytometry for the presence of CFSE high and CFSE low cells. D. Representative histogram data (for individual mice) from unvaccinated and vaccinated Wt and Spry1Flox/Flox Lck Cre mice. Percentages are representative of CFSE high and CFSE low populations. E. Graphical representation of data from five wild type and five Spry1Flox/Flox Lck Cre mice. Error bars represent one standard deviation of the mean. P values indicate statistical significance by student t-test. All experiments were performed at least three times.