Humanization and Characterization of an Anti-Human TNF-α Murine Monoclonal Antibody
Figure 4
Neutralization of TNF-α-mediated cytotoxicity in L929 cells by m357 and h357 antibodies.
Various concentrations of m357 (•) and h357 (○) antibodies were added to L929 cells cultured with 100 ng/ml of recombinant human TNF-α. The cells were incubated for 16 hrs at 37°C and cell viability was analyzed using a colorimetric MTT assay.