Flagellin lysine methyltransferase FliB catalyzes a [4Fe-4S] mediated methyl transfer reaction
Fig 4
(A) Comparison of molecular size-exclusion chromatography of ftFliB and FleBt tag carried out on a TSK-GEL BioAssist G4SWXL column. (B) SEC-MALS analysis of ftFliB. The Rayleigh ratio (light scattering, LS) profile (light green, left axis) shows one major peak. The molar mass trace (dark green, right axis) across the elution peak derived from RI/LS measurements corresponds to homogeneous oligomeric states of ftFliB. (C) 2D-AFM height profile picture of purified ftFliB oligomers in buffer that have settled on a flat MICA surface. The grayscale color profile ranges from -9 nm (black) to 9 nm (white). Scale bar is 100 nm. (D) TEM of purified ftFliB oligomers with particles of distinct globular shape enlarged below. Scale bars correspond to 25 nm.