Sperm associated antigen 9 promotes oncogenic KSHV-encoded interferon regulatory factor-induced cellular transformation and angiogenesis by activating the JNK/VEGFA pathway
Fig 4
vIRF1-upregulated SPAG9 promotes the binding of MKK4 to JNK1/2 to activate the JNK1/2 signaling.
(A). Western-blotting analysis of the expression levels of SPAG9, phosphorylated JNK1/2 and total JNK1/2 in vIRF1-expressing HUVECs. (B). Western-blotting analysis of phosphorylated JNK1/2 expression in vIRF1-expressing HUVECs transduced with lentivirus-mediated No.1 (shSPAG9-1) and No. 2 (shSPAG9-2) shRNAs targeting SPAG9. (C). Western-blotting analysis of MKK4 expression in HUVECs transduced with lentiviral-vIRF1 or its control lentiviral-pHAGE. (D) and (E). Immunoprecipitation analyses of the interaction of JNK1/2-SPAG9-MKK4 complex in vIRF1-transduced or its control pHAGE-transduced HUVECs.