The Timing of Stimulation and IL-2 Signaling Regulate Secondary CD8 T Cell Responses
Fig 4
The timing of stimulation modulates the function of 2° M CD8 T cells.
A) Total numbers of 2° M Thy1.1 P14 CD8 T cells in mL of PBL, and in the spleen, lung, and LN of individual mice from ‘early’ and ‘late’ group of mice 7 months after the initiation of the experiment. B) Representative dot plots showing cytokine production by 2° M P14 CD8 T cells, isolated from the spleen of individual mice after short ex vivo incubation in the presence of GP33 peptide. Numbers represent the percentage of 2° M P14 CD8 T cells that were positive for IFNγ and IL-2. C) Percentage of IFNγ producing 2° M P14 CD8 T cells that co-produce IL-2. Dots represent individual mice and the line represents the mean. D) Experimental design, 2° M Thy1.1 P14 CD8 T cells from ‘early’ and ‘late’ groups of mice were isolated on day 260 after transfer by positive selection and transferred in equal numbers (1.5x104 cells/mouse, i.v.) into naïve B6 Thy1.2/1.2 recipients 1 day before Att LM-GP33 (1x107 CFU/mouse i.v.) infection. E) Representative histograms showing the expression of the molecules CD27, CD62L, and KLRG1 on transferred 2° M P14 CD8 T cells from ‘early’ and late’ groups of mice. Shaded graphs represent isotype control staining and open graphs represent specific Ab staining on gated 2° M Thy1.1 P14 CD8 T cells. F) Representative dot plots showing 3° expansion of P14 CD8 T cells on day 6 after infection with Att LM-GP33. Numbers indicate the percentage of P14 CD8 T cells in the PBL. G) Percentage of P14 CD8 T cells in the PBL of individual mice on day 6 is shown. Dots represent individual mice and the line represents the mean. Data are of 5 mice per group and representative of 2–3 independent experiments. The p values are indicated; ns- not significant.