Aspalathus linearis suppresses cell survival and proliferation of enzalutamide-resistant prostate cancer cells via inhibition of c-Myc and stability of androgen receptor
Fig 4
Western blotting analysis of signaling proteins affected by GRT treatment in C4-2 MDV3100r cells.
The C4-2 MDV3100r cells were treated with increasing concentrations of GRT (0, 50, 75, and 100 μg/mL) for 48 h. Expression of Akt, PI3K p110α, PI3K p110β, PI3K p110γ, PDK1, phospho-PDK1 (S241), Skp2, cyclin D1, AR, phospho-AR (S81), Cdk1, Myc, Bcl-2, caspase 3 and PARP proteins were determined by Western blotting. Expression level of GAPDH and β-Actin were used as loading control.