RESOLFT Nanoscopy of Fixed Cells Using a Z-Domain Based Fusion Protein for Labelling
Fig 4
Immunolabelling with an M-rsEGFP2tandem fusion protein.
Maximum intensity projections of confocal microscopy z-stacks of methanol fixed CV-1 cells immunolabelled with antibodies against β-actin (A) and vimentin (B). The purified recombinant fusion protein M-rsEGFP2tandem was used to decorate the primary antibodies (red). Nuclei were labelled with DAPI (blue). Scale bars: 50 μm.