In Vivo Imaging of Hedgehog Pathway Activation with a Nuclear Fluorescent Reporter
Figure 4
Visualization of Hh signaling during adult fin regeneration.
Caudal fin of a Tg(-2.4shha:GFP-ABC;8xGliBS:mCherry-NLS) zebrafish three days after amputation. Brightfield (A) and fluorescence micrographs (B–C) are shown, with the dashed boxes corresponding to the magnified views below. Fin orientations: lateral views, anterior left. Scale bars: A–C, 2 mm; B′–C′, 200 µm.