Broad-Spectrum Transgenic Resistance against Distinct Tospovirus Species at the Genus Level
Figure 4
Phylogenetic relationship of tospoviruses based on the sequences of nucleocapsid (N) proteins.
The dendrogram was generated using the Neghbour-Joining algorithm based on 1000 bootstrap replicates. Viruses sharing close serological relationship in their N proteins are highlighted and designated as a clade. The viruses used to evaluate the transgenic resistance in this investigation are asterisked. The sequences of N genes were retrieved from GenBank of National Center for Biotechnology Information. ANSV, Alstroemeria necrotic streak virus (GQ478668); BeNMV, Bean necrosis mosaic virus (NC_018071); CaCV, Capsicum chlorosis virus (NC_008301); CCSV, Calla lily chlorotic spot virus (AY867502); CSNV, Chrysanthemum stem necrosis virus (AB600873); GBNV, Groundnut bud necrosis virus (U27809); GCFSV, Groundnut chlorotic fan-spot virus (AF080526); GRSV, Groundnut ringspot virus (L12048); GYSV, Groundnut yellow spot virus (AF013994); HCRV, Hippeastrum chlorotic ringspot virus (KC290943); INSV, Impatiens necrotic spot virus (NC_003624); IYSV, Iris yellow spot virus (AF001387); MeSMV, Melon severe mosaic virus (EU275149); MYSV, Melon yellow spot virus (AB038343); PNSV, Pepper necrotic spot virus (HE584762); PolRSV, Polygonum ringspot virus (EF445397); SVNaV, Soybean vein necrosis-associated virus (JF808214); TCSV, Tomato chlorotic spot virus (S54325); TNRV, Tomato necrotic ringspot virus (FJ489600); TSWV, Tomato spotted wilt virus (D13926); TYRV, Tomato yellow ring virus (AY686718); TZSV, Tomato zonate spot virus (NC_010489); WBNV, Watermelon bud necrosis virus (EU249351); WSMoV, Watermelon silver mottle virus (U78734); and ZLCV, Zucchini lethal chlorosis virus (AF067069).