Baicalin Protects the Cardiomyocytes from ER Stress-Induced Apoptosis: Inhibition of CHOP through Induction of Endothelial Nitric Oxide Synthase
Figure 4
Baicalin reduced Tm-induced CHOP expression in cardiomyocytes.
(A, C) Cardiomyocytes were treated with Tm (100 ng/ml) in the presence or absence of baicalin (BC) for 24 h at indicated concentrations (12.5, 25, 50 µM). (B, D) Cardiomyocytes were treated with Tm (100 ng/ml) in the presence or absence of baicalin (BC,25 µM) for 24 h at indicated times (0, 18 h, 36 h). (A, B) Representative immunoblotting and quantitative assay of phosphorylation of JNK protein expressions. (C, D) Western blot analysis and quantitative assay of CHOP protein expression. β-actin or total JNK served as loading control. *P<0.05 vs Ctrl, **P<0.05 vs Tm, n = 5.