Identification of a Live Attenuated Vaccine Candidate for Tularemia Prophylaxis
Figure 4
Immunization with the FTL_0325 mutant induces an early pro-inflammatory cytokine response in lungs.
BALB/c mice (n = 4) were infected with 1×107 CFU of the indicated mutants and wild type F. tularensis LVS. At the indicated times, the levels of pro-inflammatory cytokines were measured in lung homogenates using a Cytometric Bead Array assay. The data are representative of two independent experiments conducted and were analyzed using ANOVA with Tukey-Kramer Multiple Comparison post-test and P values were recorded. **P<0.01; ***P<0.001. Ψ = Mice infected with 1×107 CFU of F. tularensis LVS succumbed to infection by day 7 PI and hence were unavailable for comparison.