APRIL Induces Tumorigenesis and Metastasis of Colorectal Cancer Cells via Activation of the PI3K/Akt Pathway
Figure 4
APRIL stimulates the PI3K/Akt pathway in CRC cells.
(A) SW480 cells were transfected with shAPRIL (sh637) and shNTC for 48 h and analyzed for Akt and m-TOR phosphorylation by western blot. (B) HCT-116 cells were treated with the indicated concentrations of rhAPRIL for three hours and were analyzed for expression of phosphorylation of Akt and mTOR. (C) HCT-116 cells were treated with the indicated doses of LY294002 for 24 h, and then stimulated with rhAPRIL (400 ng/ml) for three hours, and analyzed for Akt and mTOR phosphorylation. The phosphorylation of Akt and mTOR was determined by SDS-PAGE and western blotting. Densitometric values are listed below each blot.