Geographical distribution and genetic diversity of Plasmodium vivax reticulocyte binding protein 1a correlates with patient antigenicity
Fig 4
Humoral immune response against PvRBP1a-N and PvRBP1a-C.
(A) Purity confirmation by SDS-PAGE of recombinant PvRBP1a-N (157–650 aa.) (64.8 kDa) and PvRBP1a-C (606–962 aa.) (48.3 kDa) expression. (B) Total IgG prevalence of each domain with the vivax patient: Myanmar (grey dot), ROK (blue dot), and Sudan (pink dot) and healthy individual (yellow dot) sera. The bar indicates the mean fluorescence intensity (MFI) ± 95% CI. The p values were calculated by Student’s t-test. Significant differences are shown as triple asterisks p <0.001 and quadruple asterisk p <0.0001. (C) IgG prevalence visualized for comparison between N and C with each patient sera by normalized reactivity index. Significant differences are shown as single asterisks p <0.05 and quadruple asterisks p <0.0001. (D) Correlation between N and C total IgG reactive indices using Pearson correlation test (ρ). Blue dot and dash line represent patient sera reactive index from ROK and its regression line. Grey and pink dot and dash line represent reactivity indices and its regression lines from Myanmar and Sudan patient sera, respectively. Red line indicates total regression.