Genome-Wide Association Analysis in Asthma Subjects Identifies SPATS2L as a Novel Bronchodilator Response Gene
Figure 4
Effect of siRNA-mediated SPATS2L knockdown on β2-adrenergic receptor levels in HASM cells.
(A) Knockdown efficiency of two SPATS2L siRNAs. Quantitative real-time PCR was done on RNAs extracted from HASM cells transfected with control non-targeting (NT) or SPATS2L-specific siRNAs. (B) Western blot analysis of β2AR protein in SPATS2L knockdown HASM cells. Three independent experiments (siRNA transfection and Western blot analysis) were done in HASM cells. The upper panel is a representative of the three Western blots. Quantification of the β2AR protein amount (normalized against the control β-actin protein) from three Western blots is shown in the lower panel.