Multiple origins and modularity in the spatiotemporal emergence of cerebellar astrocyte heterogeneity
Fig 4
Quantitative analysis of clone dispersion along the M-L and A-P axes.
(A,B) The M-L dispersion of clones tagged at E12 (green) or E14 (orange) is estimated as the longest intraclone cell distance along the M-L axis. (A) Scatterplots show that dispersion greatly decreases in E14-P30 clones compared to E12-P30 clones. HomCs are mostly found in a single cerebellar section, whereas HetCs are much more dispersed. (B) Among HetCs, triple clones are more expanded than double clones in both populations. Namely, E12-P30 triple clones are the most dispersed. (C,D) Correlation analysis shows a positive correlation between clonal size and M-L dispersion in both E12-P30 (C, n = 254 clones) and E14-P30 (D, n = 92 clones) populations (P < 0.001). (E) Distribution along the A-P axis of E12-P30 (green) and E14-P30 (orange) clones found in lobules. HomCs and double clones tagged at E12 mostly settle in a single lobule, whereas triple clones are often found in more than one lobule. E14-P30 clones are overall found in one lobule. Within lobules, cortical cells of HetCs were mostly found on the same side (i.e., lobular wall) divided by the WM (67% and 86% of E12-P30 and E14-P30 clones, respectively). *, P < 0.05; ***, P < 0.001; P values are calculated with GEE analysis. n = number of clones. The numerical data used in the figure are included in S1 Data. A-P, antero-posterior; BG, Bergmann glia; E, embryonic day; GEE, generalized estimating equations; GLA, granular layer astrocyte; HetC, heterogeneous clone; HomC, homogeneous clone; M-L, medio-lateral; WM, white matter; WMA, white matter astrocyte.