Proteome expression profiling of red blood cells during the tumorigenesis of hepatocellular carcinoma
Fig 3
Analysis of erythroid-specific protein expression in a cohort of patients with HCC, LC, and HC.
A–D. Analysis of globins expression changes in the cohort of patients with LC (n = 17), HCC (n = 30), and HC (n = 25). The expression was derived from DIA mass spectrometry data. E–F. The expression of HBA1 and HBE1 in RBCs during tumorigenesis was verified using the PRM strategy in another batch of clinical samples from LC (n = 9), HCC (n = 11), and HC (n = 10). G. Analysis of GYPA expression changes in the cohort of patients with LC (n = 17), HCC (n = 30), and HC (n = 25). H. Nucleated erythroid cells from HCC patients were observed under a microscope. I–J. Statistics of RBCs respectively expressing HbF (I) and HbA (J) in peripheral blood samples from HCC (n = 8), LC (n = 9), and HC (n = 7). The cell number was calculated by flow cytometry analysis. Statistical results were analyzed using the Wilcoxon rank-sum test. ***, P < 0.001; **, P < 0.01, *, P < 0.05.