Aspalathus linearis suppresses cell survival and proliferation of enzalutamide-resistant prostate cancer cells via inhibition of c-Myc and stability of androgen receptor
Fig 3
GRT treatment reduced soft agar colony formation of C4-2 MDV3100r cells and induced apoptosis.
(A) Anticancer activity of GRT on C4-2 MDV3100r cells was confirmed by measuring the formation of colonies on soft agar for C4-2 MDV3100r cells being treated with GRT (0, 50, 75 and 100 μg/mL, being added into the medium). The culture medium was changed twice a week for four weeks. (B) DNA damage caused by GRT treatment in C4-2 MDV3100r cells was determined using the Comet assay. Green fluorescent indicated broken DNA fragments in C4-2 MDV3100r cells being treated with increasing concentration of GRT (0, 50, 75, 100 μg/ml). (C) Tail extent moment of C4-2 MDV3100r cells in Comet assay was quantified. Data wa presented as mean ± SD in triplicate experiments. Asterisk ** and *** represents statistically significant difference p < 0.01 and p < 0.001, respectively, between the two groups of cells being compared.