Armadillo repeat-containing protein 1 is a dual localization protein associated with mitochondrial intermembrane space bridging complex
Fig 3
Levels of ArmC1 in the mitochondria of the cells depleted of the MICOS/MIB complex components.
Mitochondria were isolated from the cells where a shRNA-mediated knockdown of specific proteins was induced by the addition of doxycycline (Dox) for seven days. 25 and 50 μg of mitochondrial protein was analyzed by SDS-PAGE and western blot using antibodies against SDHA, Sam50, Mic60/Mitofilin, Mic19/CHCHD3, Mic25/CHCHD6, Mic23/ApoO and ArmC1. SDHA, Succinate dehydrogenase complex subunit A, Sam50, sorting and assembly machinery 50, CHCHD, coiled-coil-helix-coiled-coil-helix domain, ApoO, Apolipoprotein O.