Microscale spatial analysis provides evidence for adhesive monopolization of dietary nutrients by specific intestinal bacteria
Fig 3
Bifidobacterium accumulated onto starch granules in broad regions of the murine intestinal tract.
(a, b) Cross section of murine jejunum stained by Bif153 (green), Eub338 (red), Lab158 (blue), and Lugol’s solution. Bif153-positive bifidobacteria preferentially colonized onto starch granules (arrowhead) in the presence of jejunal-dominant lactic acid bacteria. (c,d) Cross section of murine colon stained by Bif153 (green), Erec482 (red), Clept1240 (blue), and Lugol’s solution. Similar to the jejunum, Bif153-positive bifidobacteria preferentially colonized starch granule surfaces (arrowhead) in the presence of colonic-dominant bacteria, Clostridium cluster XIVa and XIVb and Clostridium leptum subgroup. Images are representative of at least 7 individual mice. (a,c) Fluorescence microscopy. (b,d) Bright-field microscopy. Bars = 20 μm.