Annexin A1 on the Surface of Early Apoptotic Cells Suppresses CD8+ T Cell Immunity
Figure 3
Annexin A1 influences DC-mediated T cell stimulation.
(A) Immature DC were incubated with annexin A1 (AnxA1, 20 µg/ml), apoptotic Jurkat T cells (aJ), or left untreated. 2 days after stimulation by R-848, autologous T cells together with SEB in the indicated concentrations were added to the culture. The concentration of IFN-γ in culture supernatants was determined by ELISA after 6 days. Error bars represent means +/− SD of quadruplicate cultures. (B) For several donors, the relative IFN-γ secretion of cultures treated as in (A) is shown. IFN-γ-secretion in cultures with treated DC was calculated relative to cultures with DC stimulated by R-848 only, which was set to 100%. Error bars represent means +/− SD (n = 7). ***P<0.001. (C) Cocultures were set up as in (A) and concentrations of IL-10 in culture supernatants were determined by ELISA after 4 days. Error bars represent means +/− SD of triplicate cultures. Data are representative of at least 3 independent experiments. In all experiments, R-848 was used at a concentration of 5 or 10 µg/ml.