Blood-Brain Barrier Breakdown after Embolic Stroke in Rats Occurs without Ultrastructural Evidence for Disrupting Tight Junctions
Figure 3
Ratio of occludin-positive vessels in areas of FITC-albumin leakage.
(A) Quantitative analysis of differences in the expression of occludin in areas of FITC-albumin extravasation and their corresponding control areas was performed using low power (10× objective) magnification. Here, laminin-immunolabeling revealed the total number of vessels, whereas occludin-immunoreactivity visualized a critical tight junction constituent. The ratio of occludin-positive vessels to the total number of vessels was determined in 5 animals. (B) The ratio of occludin-positive vessels to the total number of vessels did not differ significantly in areas of FITC-albumin leakage and their corresponding control areas. Bars represent means and added lines indicate standard errors.