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Ischemic Preconditioning Increases Endothelial Progenitor Cell Number to Attenuate Partial Nephrectomy-Induced Ischemia/Reperfusion Injury

Figure 3

Double immunofluorescence staining to show the presence of EPCs in the ischemic kidney at 12 h after reperfusion.

Cellular co-expression of CD34 (red) and flk (green) in kidney medulla indicating the presence of EPCs. In Sham tissues (A–C), there was no or only slight expression of EPCs. PN (D–F) caused higher fluorescence intensity of EPCs in renal medulla. IPC (G–I) caused significant increases in the intensity of fluorescence of EPCs. [Magnification: ×200 (A–I), ×400 (J, K)].

Figure 3

doi: https://doi.org/10.1371/journal.pone.0055389.g003