Extensive Regulation of Diurnal Transcription and Metabolism by Glucocorticoids
Fig 3
Diurnal metabolite patterns show differential dependence on glucocorticoids.
(A, B) Principal component analysis (PCA) score plots of the NMR spectra of untreated samples (A) and of DEX treated together with control samples (B). (C-E) Left: Temporal profiles of metabolite levels determined by UPLC-FLR for the indicated phenotype/treatment combinations. Lines fitted by linear regression if statistical models indicate rhythmicity for wild-type (black) and rx3 strong mutant samples (white). Rhythmic model fits are indicated as continuous lines, non-rhythmic fits as broken lines. Error bars represent SEM. Right: Tukey boxplots depicting metabolite levels at all examined time points in wild-type siblings (WT, white) and rx3 strong mutants (RX3, grey) in the absence or presence of DEX.