PCNA Ubiquitination Is Important, But Not Essential for Translesion DNA Synthesis in Mammalian Cells
Figure 3
TLS in PcnaK164R/K164R mouse embryo fibroblasts.
(A) MEFs were assayed for TLS as described under Materials and Methods, using the indicated site-specific lesions. TLS extents were given as percentage relative to TLS assayed with isogenic wild type MEFs. Average results of at least three independent experiments are presented. Error bars represent standard deviations. The detailed data are presented in Table S1. (B) Percentage of accurate and mutagenic TLS across a TT CPD in Pcna+/+ and PcnaK164R/K164R MEFs. (C) Percentage of accurate and mutagenic TLS across TT 6-4 PP. The mutation types shown are: targeted (opposite the lesion), semi-targeted (at the nucleotides flanking the lesion), and mixed (both targeted and semi-targeted). (D) Percentage of accurate and mutagenic TLS across a cisPt-GG adduct. The percentage of events is calculated out of all TLS events. The detailed mutational spectra are presented in Table S2.